Protein kinase C binding protein 1 inhibits hypoxia-inducible factor-1 in the heart.

ABSTRACT:

Aims

Hypoxia-inducible factor-1 alpha (HIF-1α) is a key transcription factor responsible for the induction of genes that facilitate adaptation to hypoxia. To study HIF-1 signalling in the heart, we developed a mouse model in which an oxygen-stable form of HIF-1α can be inducibly expressed in cardiac myocytes, under the regulation of tetracycline.

Methods and results

Remarkably, expression of the transgene in mice generated two distinct phenotypes. One was the expected expression of HIF-regulated transcripts and associated changes in cardiac angiogenesis and contractility. The other was an unresponsive phenotype with much less expression of typical HIF-response genes and substantial expression of a zinc-finger protein, Protein Kinase C Binding Protein 1 (PRKCBP1). We have dem

SUBMITTER: Schunke KJ 

PROVIDER: S-EPMC6587917 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature