Detection and accurate false discovery rate control of differentially methylated regions from whole genome bisulfite sequencing.

ABSTRACT: With recent advances in sequencing technology, it is now feasible to measure DNA methylation at tens of millions of sites across the entire genome. In most applications, biologists are interested in detecting differentially methylated regions, composed of multiple sites with differing methylation levels among populations. However, current computational approaches for detecting such regions do not provide accurate statistical inference. A major challenge in reporting uncertainty is that a genome-wide scan is involved in detecting these regions, which needs to be accounted for. A further challenge is that sample sizes are limited due to the costs associated with the technology. We have developed a new approach that overcomes these challenges and assesses uncertainty for differentially methyl

SUBMITTER: Korthauer K 

PROVIDER: S-EPMC6587918 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature