Glutathione peroxidase-1 inhibits transcription of regenerating islet-derived protein-2 in pancreatic islets.

ABSTRACT: Our group previously demonstrated that overexpression of selenium-dependent glutathione peroxidase-1 (GPX1) in mice (OE) led to escalated glucose-stimulated insulin secretion and hyperinsulinemia. Because we found a strong correlation of this phenotype with a diminished expression of regenerating islet-derived protein 2 (REG2) in the OE pancreatic islets, the present study was to reveal underlying mechanisms for that down-regulation of REG2 by GPX1 as a major scavenger of reactive oxygen species. We first treated the OE and wild-type (WT) mice and their islets with ROS-generating diquat, streptozotocin, and H₂O₂ and ROS-scavenging ebselen and N-acetylcysteine (NAC). Their effects on pancreatic and islet REG2 protein and(or) secretion were opposite (P < 0.05). Thereaft

SUBMITTER: Yun JW 

PROVIDER: S-EPMC6588445 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature