Hypoxia Prevents Mitochondrial Dysfunction and Senescence in Human c-Kit⁺ Cardiac Progenitor Cells.

ABSTRACT: Senescence-associated dysfunction deleteriously affects biological activities of human c-Kit⁺ cardiac progenitor cells (hCPCs), particularly under conditions of in vitro culture. In comparison, preservation of self-renewal and decreases in mitochondrial reactive oxygen species (ROS) are characteristics of murine CPCs in vivo that reside within hypoxic niches. Recapitulating hypoxic niche oxygen tension conditions of ∼1% O₂ in vitro for expansion of hCPCs rather than typical normoxic cell culture conditions (21% O₂ ) could provide significant improvement of functional and biological activities of hCPCs. hCPCs were isolated and expanded under permanent hypoxic (hCPC-1%) or normoxic (hCPC-21%) conditions from left ventricular tissue explants collected during l

SUBMITTER: Korski KI 

PROVIDER: S-EPMC6588544 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature