A quantitative Streptococcus pyogenes-human protein-protein interaction map reveals localization of opsonizing antibodies.

ABSTRACT: A fundamental challenge in medical microbiology is to characterize the dynamic protein-protein interaction networks formed at the host-pathogen interface. Here, we generate a quantitative interaction map between the significant human pathogen, Streptococcus pyogenes, and proteins from human saliva and plasma obtained via complementary affinity-purification and bacterial-surface centered enrichment strategies and quantitative mass spectrometry. Perturbation of the network using immunoglobulin protease cleavage, mixtures of different concentrations of saliva and plasma, and different S. pyogenes serotypes and their isogenic mutants, reveals how changing microenvironments alter the interconnectivity of the interaction map. The importance of host immunoglobulins for the interaction with human

SUBMITTER: Happonen L 

PROVIDER: S-EPMC6588558 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature