A Simple Procedure for Creating Scalable Phenotypic Screening Assays in Human Neurons.

ABSTRACT: Neurons created from human induced pluripotent stem cells (hiPSCs) provide the capability of identifying biological mechanisms that underlie brain disorders. IPSC-derived human neurons, or iNs, hold promise for advancing precision medicine through drug screening, though it remains unclear to what extent iNs can support early-stage drug discovery efforts in industrial-scale screening centers. Despite several reported approaches to generate iNs from iPSCs, each suffer from technological limitations that challenge their scalability and reproducibility, both requirements for successful screening assays. We addressed these challenges by initially removing the roadblocks related to scaling of iNs for high throughput screening (HTS)-ready assays. We accomplished this by simplifying the production

SUBMITTER: Sridharan B 

PROVIDER: S-EPMC6588600 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature