Label-free detection of nanoparticles using depth scanning correlation interferometric microscopy.

ABSTRACT: Single particle level visualization of biological nanoparticles such as viruses and exosomes is challenging due to their small size and low dielectric contrast. Fluorescence based methods are highly preferred, however they require labelling which may perturb the functionality of the particle of interest. On the other hand, wide-field interferometric microscopy can be used to detect sub-diffraction limited nanoparticles without using any labels. Here we demonstrate that utilization of defocused images enhances the visibility of nanoparticles in interferometric microscopy and thus improves the detectable size limit. With the proposed method termed as Depth Scanning Correlation (DSC) Interferometric Microscopy, we experimentally demonstrate the detection of sub-35nm dielectric particles witho

SUBMITTER: Aygun U 

PROVIDER: S-EPMC6588623 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature