Quantitative Mapping of Triacylglycerol Chain Length and Saturation Using Broadband CARS Microscopy.

ABSTRACT: Lipid droplets (LDs), present in many cell types, are highly dynamic organelles that store neutral lipids, primarily triacylglycerols (TAGs). With the discovery of new LD functions (e.g., in immune response, protein clearage, and occurrence with disease), new methods to study LD chemical composition in situ are necessary. We present an approach for in situ, quantitative TAG analysis using label-free, coherent Raman microscopy that allows deciphering LD TAG composition in different biochemically complex samples with submicrometer spatial resolution. Employing a set of standard TAGs, we generate a spectral training matrix capturing the variation caused in Raman-like spectra by TAG backbone, chain length, and number of double bonds per chain, as well as the presence of proteins or other dilut

SUBMITTER: Paul A 

PROVIDER: S-EPMC6588730 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature