Electrochemical patterns during Drosophila oogenesis: ion-transport mechanisms generate stage-specific gradients of pH and membrane potential in the follicle-cell epithelium.

ABSTRACT:

Background

Alterations of bioelectrical properties of cells and tissues are known to function as wide-ranging signals during development, regeneration and wound-healing in several species. The Drosophila follicle-cell epithelium provides an appropriate model system for studying the potential role of electrochemical signals, like intracellular pH (pH_i) and membrane potential (V_mem), during development. Therefore, we analysed stage-specific gradients of pH_i and V_mem as well as their dependence on specific ion-transport mechanisms.

Results

Using fluorescent indicators, we found distinct alterations of pH_i- and V_mem-patterns during stages 8 to 12 of oogenesis. To determine the roles of relevant ion-transport mecha

SUBMITTER: Weiß I 

PROVIDER: S-EPMC6588877 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature