Prostaglandin E₂ inhibits profibrotic function of human pulmonary fibroblasts by disrupting Ca²⁺ signaling.

ABSTRACT: We have shown that calcium (Ca²⁺) oscillations in human pulmonary fibroblasts (HPFs) contribute to profibrotic effects of transforming growth factor-β (TGF-β) and that disruption of these oscillations blunts features of pulmonary fibrosis. Prostaglandin E₂ (PGE₂) exerts antifibrotic effects in the lung, but the mechanisms for this action are not well defined. We thus sought to explore interactions between PGE₂ and the profibrotic agent TGF-β in pulmonary fibroblasts (PFs) isolated from patients with or without idiopathic pulmonary fibrosis (IPF). PGE₂ inhibited TGF-β-promoted [Ca²⁺] oscillations and prevented the activation of Akt and Ca²⁺/calmodulin-dependent protein kinase-II (CaMK-II) but did not prevent activa

SUBMITTER: Mukherjee S 

PROVIDER: S-EPMC6589580 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature