ANO1/TMEM16A regulates process maturation in radial glial cells in the developing brain.

ABSTRACT: Neural stem cells (NSCs) are primary progenitor cells in the early developmental stage in the brain that initiate a diverse lineage of differentiated neurons and glia. Radial glial cells (RGCs), a type of neural stem cell in the ventricular zone, are essential for nurturing and delivering new immature neurons to the appropriate cortical target layers. Here we report that Anoctamin 1 (ANO1)/TMEM16A, a Ca²⁺-activated chloride channel, mediates the Ca²⁺-dependent process extension of RGCs. ANO1 is highly expressed and functionally active in RGCs of the mouse embryonic ventricular zone. Knockdown of ANO1 suppresses RGC process extension and protrusions, whereas ANO1 overexpression stimulates process extension. Among various trophic factors, brain-derived neurotrophic fact

SUBMITTER: Hong GS 

PROVIDER: S-EPMC6589654 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature