Dual RXR motifs regulate nerve growth factor-mediated intracellular retention of the delta opioid receptor.

ABSTRACT: The delta opioid receptor (DOR), a physiologically relevant prototype for G protein-coupled receptors, is retained in intracellular compartments in neuronal cells. This retention is mediated by a nerve growth factor (NGF)-regulated checkpoint that delays the export of DOR from the trans-Golgi network. How DOR is selectively retained in the Golgi, in the midst of dynamic membrane transport and cargo export, is a fundamental unanswered question. Here we address this by investigating sequence elements on DOR that regulate DOR surface delivery, focusing on the C-terminal tail of DOR that is sufficient for NGF-mediated regulation. By systematic mutational analysis, we define conserved dual bi-arginine (RXR) motifs that are required for NGF- and phosphoinositide-regulated DOR export from intrace

SUBMITTER: Shiwarski DJ 

PROVIDER: S-EPMC6589700 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature