Determination of hemolysis index thresholds for biochemical tests on Siemens Advia 2400 chemistry analyzer.

ABSTRACT:

Background

In vitro hemolysis is still the most common source of pre-analytical nonconformities. This study aimed to investigate the hemolytic effects on commonly used biochemical tests as well as to determine the hemolysis index (HI) thresholds on Siemens Advia 2400 chemistry analyzer.

Methods

Peripheral blood samples were collected from forty healthy volunteers. Hemolysis was achieved using syringes. Five hemolysis levels were produced including the no hemolysis group, slight hemolysis group, mild hemolysis group, moderate hemolysis group, and heavy hemolysis group. We then used the bias from baseline (no hemolysis) and HI to construct regression functions. The HI corresponding to the bias limits was considered as HI thresholds. We chose the total allowable error (TAE) as

SUBMITTER: Du Z 

PROVIDER: S-EPMC6589729 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature