Multifunctional elastin-like polypeptide renders β-glucosidase enzyme phase transition and high stability.

ABSTRACT:

Background

In the enzymatic conversion of biomass, it becomes an important issue to efficiently and cost-effectively degrade cellulose into fermentable glucose. β-Glucosidase (Bgluc), an essential member of cellulases, plays a critical role in cellulosic biomass degradation. The difficulty in improving the stability of Bgluc has been a bottleneck in the enzyme-dependent cellulose degradation. The traditional method of protein purification, however, leads to higher production cost and a decrease in activity. To simplify and efficiently purify Bgluc with modified special properties, Bgluc-tagged ELP and His with defined phase transitions was designed to facilitate the process.

Results

Here, a novel binary ELP and His tag was fused with Bgluc from termite Coptotermes formosa

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC6589881 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature