Subtype Diversification and Synaptic Specificity of Stem Cell-Derived Spinal Interneurons.

ABSTRACT: Neuronal diversification is a fundamental step in the construction of functional neural circuits, but how neurons generated from single progenitor domains acquire diverse subtype identities remains poorly understood. Here we developed an embryonic stem cell (ESC)-based system to model subtype diversification of V1 interneurons, a class of spinal neurons comprising four clades collectively containing dozens of molecularly distinct neuronal subtypes. We demonstrate that V1 subtype diversity can be modified by extrinsic signals. Inhibition of Notch and activation of retinoid signaling results in a switch to MafA clade identity and enriches differentiation of Renshaw cells, a specialized MafA subtype that mediates recurrent inhibition of spinal motor neurons. We show that Renshaw cells are int

SUBMITTER: Hoang PT 

PROVIDER: S-EPMC6590086 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature