Nanopore sequencing: An enrichment-free alternative to mitochondrial DNA sequencing.

ABSTRACT: Mitochondrial DNA sequence data are often utilized in disease studies, conservation genetics and forensic identification. The current approaches for sequencing the full mtGenome typically require several rounds of PCR enrichment during Sanger or MPS protocols followed by fairly tedious assembly and analysis. Here we describe an efficient approach to sequencing directly from genomic DNA samples without prior enrichment or extensive library preparation steps. A comparison is made between libraries sequenced directly from native DNA and the same samples sequenced from libraries generated with nine overlapping mtDNA amplicons on the Oxford Nanopore MinION™ device. The native and amplicon library preparation methods and alternative base calling strategies were assessed to establish error rates

SUBMITTER: Zascavage RR 

PROVIDER: S-EPMC6590251 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature