DEPArray™ system: An automatic image-based sorter for isolation of pure circulating tumor cells.

ABSTRACT: Circulating tumor cells (CTCs) are rare cells shed into the bloodstream by invasive tumors and their analysis offers a promising noninvasive tool to predict and monitor therapeutic responses. CTCs can be isolated from patient blood and their characterization at single-cell level can inform on the genomic landscape of a tumor. All CTC enrichment methods bear a burden of contaminating normal cells, which mandate a further step of purification to enable reliable downstream genetic analysis. Here, we describe the DEPArray™ technology, a microchip-based digital sorter, which combines precise microfluidic and microelectronic enabling precise, image-based isolation of single CTCs, which can then be analyzed by Next Generation Sequencing (NGS) methods. © 2018 The Authors. Cytometry Part A publishe

SUBMITTER: Di Trapani M 

PROVIDER: S-EPMC6590341 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature