Cloning of carrier cells infected with oncolytic adenovirus driven by midkine promoter and biosafety studies.

ABSTRACT:

Background

A549 carrier cells infected with oncolytic adenovirus can induce complete tumor reduction of subcutaneous ovarian tumors but not intraperitoneal disseminated ovarian tumors. This appears to be a result of the insufficient antitumor effect of A549 carrier cells. Therefore, in the present study, we cloned a novel carrier cell with the aim of improving the antitumor effects.

Methods

Carrier cells infected with oncolytic adenovirus AdE3-midkine with a midkine promoter were cloned by limiting dilution. We examined the antitumor effects of these cells on subcutaneous and intraperitoneal OVHM ovarian tumors in a syngeneic mouse model. Biosafety tests were conducted in beagle dogs and rabbits.

Results

We cloned EHMK-51-35 carrier cells with 10-fold higher antitumor

SUBMITTER: Hamada K 

PROVIDER: S-EPMC6590659 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature