Mechanism of cholesterol efflux in humans after infusion of reconstituted high-density lipoprotein.

ABSTRACT:

Objectives

Infusion of reconstituted HDL (rHDL) leads to changes in HDL metabolism as well as to an increased capacity of plasma to support cholesterol efflux providing an opportunity to investigate mechanisms linking cholesterol efflux to changes in plasma HDL.

Methods and results

Patient plasmas after infusion of rHDL were tested ex vivo for their capacity to stimulate cholesterol efflux. Reconstituted HDL enhanced mobilization of cholesterol from tissues in vivo as shown by rising HDL cholesterol concentrations over the infusion period. Infusion of rHDL in vivo led to increased cholesterol efflux ex vivo; surprisingly, removing apoB-containing lipoproteins while preserving all HDL subfractions eliminated this increase. Infusion of rHDL led to the remodelling of plasma HDL

SUBMITTER: Hoang A 

PROVIDER: S-EPMC6590868 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature