Heterozygous IDH1^R132H/WT created by "single base editing" inhibits human astroglial cell growth by downregulating YAP.

ABSTRACT: Mutations in the isocitrate dehydrogenase 1 (IDH1) gene have been identified in a number of cancer types, including brain cancer. The Cancer Genome Atlas project has revealed that IDH1 mutations occur in 70-80% of grade II and grade III gliomas. Until recently, most of the functional studies of IDH1 mutations in cellular models have been conducted in overexpression systems with the IDH1 wild type background. In this study, we employed a modified CRISPR/Cas9 genome editing technique called "single base editing", and efficiently introduced heterozygous IDH1 R132H mutation (IDH1^R132H/WT) in human astroglial cells. Global DNA methylation profiling revealed hypermethylation as well as hypomethylation induced by IDH1^R132H/WT. Global gene expression analysis identified molec

SUBMITTER: Wei S 

PROVIDER: S-EPMC6590918 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature