Semaphorin 4A Stabilizes Human Regulatory T Cell Phenotype via Plexin B1.

ABSTRACT: We previously reported that neuroimmune semaphorin (Sema) 4A regulates the severity of experimental allergic asthma and increases regulatory T (Treg) cell numbers in vivo; however, the mechanisms of Sema4A action remain unknown. It was also reported that Sema4A controls murine Treg cell function and survival acting through neuropilin 1 (NRP-1) receptor. To clarify Sema4A action on human T cells, we employed T cell lines (HuT78 and HuT102), human PBMCs, and CD4⁺ T cells in phenotypic and functional assays. We found that HuT78 demonstrated a T effector-like phenotype (CD4⁺CD25^lowFoxp3^-), whereas HuT102 expressed a Treg-like phenotype (CD4⁺CD25^hi Foxp3⁺). Neither cell line expressed NRP-1. HuT102 cells expressed Sema

SUBMITTER: Chapoval SP 

PROVIDER: S-EPMC6590919 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature