Phosphatidylinositol 5 Phosphate 4-Kinase Regulates Plasma-Membrane PIP₃ Turnover and Insulin Signaling.

ABSTRACT: Phosphatidylinositol 3,4,5-trisphosphate (PIP₃) generation at the plasma membrane is a key event during activation of receptor tyrosine kinases such as the insulin receptor required for normal growth and metabolism. We report that in Drosophila, phosphatidylinositol 5 phosphate 4-kinase (PIP4K) is required to limit PIP₃ levels during insulin receptor activation. Depletion of PIP4K increases the levels of PIP₃ produced in response to insulin stimulation. We find that PIP4K function at the plasma membrane enhances class I phosphoinositide 3-kinase (PI3K) activity, although the catalytic ability of PIP4K to produce phosphatidylinositol 4,5-bisphosphate [PI(4,5)P₂] at the plasma membrane is dispensable for this regulation. Animals lacking PIP4K show

SUBMITTER: Sharma S 

PROVIDER: S-EPMC6591132 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature