Negative Ion Mode Collision-Induced Dissociation for Analysis of Protein Arginine Methylation.

ABSTRACT: Arginine methylation is a common protein post-translational modification (PTM) that plays a key role in eukaryotic cells. Three distinct types of this modification are found in mammals: asymmetric N^η1N^η1-dimethylarginine (aDMA), symmetric N^η1N^η2-dimethylarginine (sDMA), and an intermediate N^η1-monomethylarginine (MMA). Elucidation of regulatory mechanisms of arginine methylation in living organisms requires precise information on both the type of the modified residues and their location inside the protein amino acid sequences. Despite mass spectrometry (MS) being the method of choice for analysis of multiple protein PTMs, unambiguous characterization of protein arginine methylation may not be always straightforward. Indeed, frequent in

SUBMITTER: Katsanovskaja K 

PROVIDER: S-EPMC6591203 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature