Recombinant production of human α₂-macroglobulin variants and interaction studies with recombinant G-related α₂-macroglobulin binding protein and latent transforming growth factor-β₂.

ABSTRACT: α₂-Macroglobulins (α₂Ms) regulate peptidases, hormones and cytokines. Mediated by peptidase cleavage, they transit between native, intact forms and activated, induced forms. α₂Ms have been studied over decades using authentic material from primary sources, which was limited by sample heterogeneity and contaminants. Here, we developed high-yield expression systems based on transient transfection in Drosophila Schneider 2 and human Expi293F cells, which produced pure human α₂M (hα₂M) at ~1.0 and ~0.4 mg per liter of cell culture, respectively. In both cases, hα₂M was mainly found in the induced form. Shorter hα₂M variants encompassing N-/C-terminal parts were also expressed and yielded pure material at ~1.6/~1.3 and

SUBMITTER: Marino-Puertas L 

PROVIDER: S-EPMC6591361 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature