Enabling Large-Scale Ex Vivo Production of Megakaryocytes from CD34⁺ Cells Using Gas-Permeable Surfaces.

ABSTRACT: Patients suffering from acute or sustained thrombocytopenia require platelet transfusions, which are entirely donor-based and limited by challenges related to storage and fluctuating supply. Developing cell-culture technologies will enable ex vivo and donor-independent platelet production. However, critical advancements are needed to improve scalability and increase megakaryocyte (Mk) culture productivity. To address these needs, we evaluated Mk production from mobilized peripheral blood CD34⁺ cells cultured on a commercially available gas-permeable silicone rubber membrane, which provides efficient gas exchange, and investigated the use of fed-batch media dilution schemes. Starting with a cell-surface density of 40 × 10³ CD34⁺ cells per cm² (G40

SUBMITTER: Martinez AF 

PROVIDER: S-EPMC6591548 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature