Modified Mouse Model of Clostridioides difficile Infection as a Platform for Probiotic Efficacy Studies.

ABSTRACT: Probiotics may represent a promising approach for reducing Clostridioides (Clostridium) difficile infections (CDIs). A clinical trial conducted by our group demonstrated that CDI patients undergoing adjunctive treatment with Lactobacillus and Bifidobacterium probiotics had a reduction in diarrheal duration and compositional changes in their stool microbiomes. Here, we modified a CDI mouse model to represent clinical outcomes observed in patients and employed this model to identify evidence for the prevention of primary CDI and relapse with the same probiotic. Mice (n = 80) were administered 0.25 mg/ml cefoperazone over 5 days and subsequently challenged with 102C. difficile VPI 10463 spores. A subset of mice (n = 40) were administered 108 CFU of probiotics daily alongside cefoperazone pret

SUBMITTER: De Wolfe TJ 

PROVIDER: S-EPMC6591643 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature