Use of GC-MS based metabolic fingerprinting for fast exploration of fungicide modes of action.

ABSTRACT:

Background

The widespread occurrence of fungicide resistance in fungal plant pathogens requires the development of new compounds with different mode(s) of action (MOA) to avoid cross resistance. This will require a rapid method to identify MOAs.

Results

Here, gas chromatography-mass spectrometry (GC-MS) based metabolic fingerprinting was used to elucidate the MOAs of fungicides. Botrytis cinerea, an important pathogen of vegetables and flowers, can be inhibited by a wide range of chemical fungicides with different MOAs. A sensitive strain of B. cinerea was exposed to EC₅₀ concentrations of 13 fungicides with different known MOAs and one with unknown MOA. The mycelial extracts were analyzed for their "metabolic fingerprint" using GC-MS. A comparison among the GC-MS

SUBMITTER: Hu Z 

PROVIDER: S-EPMC6591849 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature