Effect of exogenous gonadotropin on the transcriptome of human granulosa cells and follicular fluid hormone profiles.

ABSTRACT:

Background

Superovulation treatment had some adverse effects on maturity and development of oocytes. Can superovulation dose of gonadotropins (Gns) affect the transcriptome of granulosa cells and follicular fluid (FF) hormone levels?

Methods

One leading pre-ovulatory follicle per subject was used from three natural-cycle and four Gn-stimulated patients. Granulosa cells and FF samples were collected from the same leading follicle of each patient. RNA was extracted from granulosa cells and subjected to deep sequencing and analysis. Follicle-stimulating hormone (FSH), estradiol (E2), androstenedione (AND), testosterone (T), luteinizing hormone (LH), and progesterone (P4) levels in FF were measured by immunoassays. Student's t test was used for statistical analysis.

Results</

SUBMITTER: Lu CL 

PROVIDER: S-EPMC6591892 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature