Azithromycin induces epidermal differentiation and multivesicular bodies in airway epithelia.

ABSTRACT:

Background

Azithromycin (Azm) is a macrolide recognized for its disease-modifying effects and reduction in exacerbation of chronic airway diseases. It is not clear whether the beneficial effects of Azm are due to its anti-microbial activity or other pharmacological actions. We have shown that Azm affects the integrity of the bronchial epithelial barrier measured by increased transepithelial electrical resistance. To better understand these effects of Azm on bronchial epithelia we have investigated global changes in gene expression.

Methods

VA10 bronchial epithelial cells were treated with Azm and cultivated in air-liquid interface conditions for up to 22 days. RNA was isolated at days 4, 10 and 22 and analyzed using high-throughput RNA sequencing. qPCR and immunostaining wer

SUBMITTER: Arason AJ 

PROVIDER: S-EPMC6591972 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature