Protein corona formed on silver nanoparticles in blood plasma is highly selective and resistant to physicochemical changes of the solution.

ABSTRACT: Nanoparticles (NPs) in contact with protein-containing media such as biological fluids rapidly acquire a surface layer of proteins, known as the protein corona. The protein composition and structural properties of the protein corona are crucial for NP interactions with living cells. Although much has been learned about the protein corona phenomenon, further elucidation could benefit from extensive quantitative proteomics analysis. Herein we report a comprehensive quantitative characterization (>350 proteins) of the corona that formed on 60 nm silver NPs via interaction with human blood plasma, as a function of pH and temperature. By varying the pH and temperature one can access different conformational spaces and charge localizations of the plasma proteins, which in turn provide kno

SUBMITTER: Gorshkov V 

PROVIDER: S-EPMC6592156 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature