Transcriptomic Hallmarks of Tumor Plasticity and Stromal Interactions in Brain Metastasis.

ABSTRACT: The brain is a major site of relapse for several cancers, yet deciphering the mechanisms of brain metastasis remains a challenge because of the complexity of the brain tumor microenvironment (TME). To define the molecular landscape of brain metastasis from intact tissue in vivo, we employ an RNA-sequencing-based approach, which leverages the transcriptome of xenografts and distinguishes tumor cell and stromal gene expression with improved sensitivity and accuracy. Our data reveal shifts in epithelial and neuronal-like lineage programs in malignant cells as they adapt to the brain TME and the reciprocal neuroinflammatory response of the stroma. We identify several transcriptional hallmarks of metastasis that are specific to particular regions of the brain, induced across multiple tumor type

SUBMITTER: Wingrove E 

PROVIDER: S-EPMC6592283 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature