An unusual and vital protein with guanylate cyclase and P4-ATPase domains in a pathogenic protist.

ABSTRACT: cGMP signaling is one of the master regulators of diverse functions in eukaryotes; however, its architecture and functioning in protozoans remain poorly understood. Herein, we report an exclusive guanylate cyclase coupled with N-terminal P4-ATPase in a common parasitic protist, Toxoplasma gondii This bulky protein (477-kD), termed TgATPase_P-GC to fairly reflect its envisaged multifunctionality, localizes in the plasma membrane at the apical pole of the parasite, whereas the corresponding cGMP-dependent protein kinase (TgPKG) is distributed in the cytomembranes. TgATPase_P-GC is refractory to genetic deletion, and its CRISPR/Cas9-assisted disruption aborts the lytic cycle of T. gondii Besides, Cre/loxP-mediated knockdown of TgAT

SUBMITTER: Gunay-Esiyok O 

PROVIDER: S-EPMC6592433 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature