Standard Proteoforms and Their Complexes for Native Mass Spectrometry.

ABSTRACT: Native mass spectrometry (nMS) is a technique growing at the interface of analytical chemistry, structural biology, and proteomics that enables the detection and partial characterization of non-covalent protein assemblies. Currently, the standardization and dissemination of nMS is hampered by technical challenges associated with instrument operation, benchmarking, and optimization over time. Here, we provide a standard operating procedure for acquiring high-quality native mass spectra of 30-300 kDa proteins using an Orbitrap mass spectrometer. By describing reproducible sample preparation, loading, ionization, and nMS analysis, we forward two proteoforms and three complexes as possible standards to advance training and longitudinal assessment of instrument performance. Spectral data for fi

SUBMITTER: Schachner LF 

PROVIDER: S-EPMC6592724 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature