Stimulation of β-adrenoceptors up-regulates cardiac expression of galectin-3 and BIM through the Hippo signalling pathway.

ABSTRACT:

Background and purpose

Expression of the pro-fibrotic galectin-3 and the pro-apoptotic BIM is elevated in diseased heart or after β-adrenoceptor stimulation, but the underlying mechanisms are unclear. This question was addressed in the present study.

Experimental approach

Wild-type mice and mice with cardiac transgenic expression of β₂ -adrenoceptors, mammalian sterile-20 like kinase 1 (Mst1) or dominant-negative Mst1, and non-specific galectin-3 knockout mice were used. Effects of the β-adrenoceptor agonist isoprenaline or β-adrenoceptor antagonists were studied. Rat cardiomyoblasts (H9c2) were used for mechanistic exploration. Biochemical assays were performed.

Key results

Isoprenaline treatment up-regulated expression of galectin-3 and BIM, and this was

SUBMITTER: Zhao WB 

PROVIDER: S-EPMC6592853 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature