SmFRET Probing Reveals Substrate-Dependent Conformational Dynamics of E. coli Multidrug MdfA.

ABSTRACT: Bacterial multidrug-resistance transporters of the major facilitator superfamily are distinguished by their extraordinary ability to bind structurally diverse substrates, thus serving as a highly efficient tool to protect cells from multiple toxic substances present in their environment, including antibiotic drugs. However, details of the dynamic conformational changes of the transport cycle involved remain to be elucidated. Here, we used the single-molecule fluorescence resonance energy transfer technique to investigate the conformational behavior of the Escherichia coli multidrug transporter MdfA under conditions of different substrates, pH, and alkali metal ions. Our data show that different substrates exhibit distinct effects on both the conformational distribution and transition rate

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC6592872 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature