Two QTLs controlling Clubroot resistance identified from Bulked Segregant Sequencing in Pakchoi (Brassica campestris ssp. chinensis Makino).

ABSTRACT: Clubroot, caused by Plasmodiophora Brassicae, is a serious soil-borne disease in worldwide. In recent years, progression of clubroot is rapid and serious in Shanghai, China. In this study, The inheritance of clubroot resistance (CR) were determined in pakchoi using F₂ segregation population that were developed by crossing highly resistant line 'CR38' and susceptible line 'CS22'. Two novel QTLs, qBrCR38-1 and qBrCR38-2, was identified by BSA-seq (Bulked Segregant Sequencing) resistant to P. brassicae physiological race 7. Two significant peak qBrCR38-1 and qBrCR38-2 were observed by three statistical methods between interval of 19.7-20.6 Mb in chromosome A07 and 20.0-20.6 Mb in chromosome A08, respectively. In addition, Polymorphic SNPs identified within target regions were conve

SUBMITTER: Zhu H 

PROVIDER: S-EPMC6592919 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature