Dataset Information

Improved quality of samples and laboratory turnaround time using 3.5 mL low vacuum BD Vacutainer^® Barricor tubes in the emergency department.

ABSTRACT:

Background

Centrifugation is a consuming time step which participates to increase the turnaround time (TAT) in laboratories, likewise hemolysis sample that needs a re-sampling could delay management of patients. Recently, it has been postulated that BD Barricor™ tube could allow to decrease the centrifugation time and prevent hemolysis, two key feature to ensure high-quality results.Aim of the study was to evaluate the impact of replacing 4 mL BD vacutainer heparin lithium tube by low vacuum 3.5 mL BD vacutainer Barricor™ tube in an emergency department (ED) on hemolysis rate and TAT.

Methods

Data of hemolysis index (HI) and TAT were compared between the first period of 15 days using 4 mL BD vacutainer heparin lithium tubes with 15 min at 2000xg as centrifugation setting and a second period of 15 days using BD vacutainer Barricor™ tube centrifuged 3 min at 4000xg.

Results

A significantly reduced time duration between reception of sample and available results in informatics lab system was observed with the reduction time of centrifugation allowed by use of Barricor™ tube compared to regular heparin lithium tubes (p < 0.001). A significative decrease in hemolysis rate also occurred in the second period as samples with HI < 10 reached from 52.5% in the first period to 68.5% (p < 0.001) in the second.

Conclusion

Low vacuum Barricor^TM tubes allowing a higher speed of centrifugation improve lab TAT without impairment of sample quality as a significant reduction of hemolysis was observed, a double advantage which is of particular interest for ED.

SUBMITTER: Badiou S

PROVIDER: S-EPMC6593183 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Publications

Improved quality of samples and laboratory turnaround time using 3.5 mL low vacuum BD Vacutainer<sup>®</sup> Barricor tubes in the emergency department.

Badiou S S Vuillot O O Bargnoux A S AS Kuster N N Lefebvre S S Sebbane M M Cristol J P JP Dupuy A M AM

Practical laboratory medicine 20190612

<h4>Background</h4>Centrifugation is a consuming time step which participates to increase the turnaround time (TAT) in laboratories, likewise hemolysis sample that needs a re-sampling could delay management of patients. Recently, it has been postulated that BD Barricor™ tube could allow to decrease the centrifugation time and prevent hemolysis, two key feature to ensure high-quality results.Aim of the study was to evaluate the impact of replacing 4 mL BD vacutainer heparin lithium tube by low va ...[more]

PMID: 31289734

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Project description:Introduction. Clinical microbiology laboratories have had to cope with an increase in the volume of tests due to the emergence of the SARS-CoV-2 virus. Short turnaround times (TATs) are important for case tracing and to help clinicians in patient management. In such a context, high-throughput systems are essential to process the bulk of the tests. Rapid tests are also required to ensure shorter TATs for urgent situations. In our laboratory, SARS-CoV-2 assays were initially implemented on our custom platform using a previously published method. The commercial cobas 6800 (Roche diagnostics) assay and the GeneXpert Xpress (Cepheid) SARS-CoV-2 assay were implemented on 24 March and 8 April 2020, respectively, as soon as available.Hypothesis/Gap Statement. Despite the abundant literature on SARS-CoV-2 assays, the articles focus mainly on the diagnostic performances. This is to our knowledge the first article that specifically studies the TAT of different assays.Aim. We aimed to describe the impact of various SARS-CoV-2 assays on the TAT at the beginning of the outbreak.Methodology. In this study, we retrospectively analysed the TAT of all SARS-CoV-2 assays performed in our centre between 24 February and 9 June, 2020.Results. We retrieved 33 900 analyses, with a median TAT of 6.25 h. TATs were highest (6.9 h) when only our custom platform was used (24 February to 24 March, 2020). They were reduced to 6.1 h when the cobas system was introduced (24 March to 8 April, 2020). The implementation of the GeneXpert further reduced the median TAT to 4.8 h (8 April to 9 June, 2020). The GeneXpert system had the shortest median TAT (1.9 h), followed by the cobas (5.5 h) and by our custom platform (6.9 h).Conclusion. This work shows that the combination of high-throughput systems and rapid tests allows the efficient processing of a large number of tests with a short TAT. In addition, the use of a custom platform allowed the quick implementation of an in-house test when commercial assays were not yet available.

Dataset Information

Improved quality of samples and laboratory turnaround time using 3.5 mL low vacuum BD Vacutainer^® Barricor tubes in the emergency department.

Background

Methods

Results

Conclusion

Publications

Improved quality of samples and laboratory turnaround time using 3.5 mL low vacuum BD Vacutainer<sup>®</sup> Barricor tubes in the emergency department.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Improved quality of samples and laboratory turnaround time using 3.5 mL low vacuum BD Vacutainer® Barricor tubes in the emergency department.

Background

Methods

Results

Conclusion

Publications

Improved quality of samples and laboratory turnaround time using 3.5 mL low vacuum BD Vacutainer<sup>®</sup> Barricor tubes in the emergency department.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Improved quality of samples and laboratory turnaround time using 3.5 mL low vacuum BD Vacutainer^® Barricor tubes in the emergency department.

Improved quality of samples and laboratory turnaround time using 3.5 mL low vacuum BD Vacutainer<sup>®</sup> Barricor tubes in the emergency department.