Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways.

ABSTRACT: A better understanding of essential cellular functions in pathogenic bacteria is important for the development of more effective antimicrobial agents. We performed a comprehensive identification of essential genes in Mycobacterium tuberculosis, the major causative agent of tuberculosis, using a combination of transposon insertion sequencing (Tn-seq) and comparative genomic analysis. To identify conditionally essential genes by Tn-seq, we used media with different nutrient compositions. Although many conditional gene essentialities were affected by the presence of relevant nutrient sources, we also found that the essentiality of genes in a subset of metabolic pathways was unaffected by metabolite availability. Comparative genomic analysis revealed that not all essential genes identif

SUBMITTER: Minato Y 

PROVIDER: S-EPMC6593218 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature