Rapid Multiplex Genotyping of 20 HLA-A^*02:01 Restricted Minor Histocompatibility Antigens.

ABSTRACT: A subset of MHC-associated self-peptides presented by the recipient's cells and immunologically foreign to the donor can induce an allogeneic immune response after hematopoietic stem cell transplantation (HSCT). These immunogenic peptides originate from the genomic polymorphisms and are known as minor histocompatibility antigens (MiHA). MiHA mismatches trigger the post-transplant immune response, which could manifest in both the deleterious "graft-vs.-host" disease and the beneficial "graft-vs.-leukemia" effect. Importantly, some MiHAs are considered to be promising targets for posttransplant T-cell immunotherapy of hematopoietic malignancies. This creates a demand for a robust and fast approach to genotyping MiHA-encoding polymorphisms. We report a multiplex real-time PCR method for the g

SUBMITTER: Romaniuk DS 

PROVIDER: S-EPMC6593292 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature