Frequency of the Mycobacterium tuberculosis RD^Rio genotype and its association with multidrug-resistant tuberculosis.

ABSTRACT:

Background

In recent decades, Mycobacterium tuberculosis with the RD^Rio genotype, frequently isolated from tuberculosis patients in Rio de Janeiro, has become part of the Latin American - Mediterranean (LAM) family and has been associated with multidrug-resistant tuberculosis (MDR-TB). The aim of this study was to investigate the frequency of M. tuberculosis RD^Rio in the state of Minas Gerais, Brazil, and its relationship with MDR-TB.

Methods

For convenience, 172 susceptible and 63 MDR M. tuberculosis isolates were taken from pulmonary samples from patients diagnosed between January 2007 and December 2011. The DNA extracted from these isolates was analyzed by spoligotyping, PCR-RFLP to characterize fbpC¹⁰³/Ag85C103, multiplex PCR to detect RD

SUBMITTER: de Almeida IN 

PROVIDER: S-EPMC6593491 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature