Intragenic regulation of SOCS3 isoforms.

ABSTRACT:

Background

Inflammatory reactions are commonly affected by stress responses. Interleukin-6 signalling is part of the inflammatory response and is stringently regulated by the feedback inhibitor SOCS3 expressed in a short and long isoform. Here, we studied the inhibitory potential of the two SOCS3 isoforms. Furthermore, we analysed the regulation of SOCS3 isoform expression and the role of PKR stress kinase signalling in SOCS3 protein expression.

Methods

We performed Western blotting, reporter assays, genetic analyses and manipulations for studying SOCS3 isoform expression and activation of signalling components involved in interleukin-6-induced and PKR-dependent signalling.

Results

Interleukin-6-induced endogenous expression of both SOCS3 isoforms was found in distinc

SUBMITTER: Klepsch O 

PROVIDER: S-EPMC6593527 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature