Proteomic characterization of bovine granulosa cells in dominant and subordinate follicles.

ABSTRACT:

Background

Characterization of molecular factors regulating ovarian follicular development is critical to understanding its functional mechanism of controlling the estrous cycle, determining oocyte competency, and regulating ovulation. In previous studies, we performed next-gene sequencing to investigate the differentially expressed transcripts of bovine follicular granulosa cells (GCs) at the dominant follicle (DF) and subordinate follicle (SF) stages during the first follicular wave. This study aims to investigate the proteomic characterization of GCs of DF and SF in the bovine estrous cycle.

Results

In total, 3409 proteins were identified from 30,321 peptides obtained from liquid chromatograph-mass spectrometer analysis. Two hundred fifty-nine of these proteins were found

SUBMITTER: Hao Q 

PROVIDER: S-EPMC6593542 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature