Labeling of human mesenchymal stem cells with different classes of vital stains: robustness and toxicity.

ABSTRACT:

Background

Mesenchymal stem cell (MSC) transplantation has been explored as a new clinical approach to repair injured tissues. However, in order to evaluate the therapeutic activity of MSC, cell tracking techniques are required to determine the fate of transplanted cells in both preclinical and clinical studies. In these aspects, different vital stains offer the potential for labeling and monitoring of grafted cells in vivo. It is desirable to have tracking agents which have long-term stability, are not toxic to the cells, and do not affect cell function.

Methods

Here, we selected three different labels: CellTracker™ Green CMFDA, eGFP-mRNA (genetic pre-tag), and Molday ION Rhodamine B™ (nanoparticle-based fluorescent and magnetic label) and performed extensive analysis of th

SUBMITTER: Andrzejewska A 

PROVIDER: S-EPMC6593614 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature