Determination of Peptide Profile Consistency and Safety of Collagen Hydrolysates as Quality Attributes.

ABSTRACT: Collagen hydrolysates are dietary supplements used for nutritional and medical purposes. They are complex mixtures of low-molecular-weight peptides obtained from the enzymatic hydrolysis of collagen, which provide intrinsic batch-to-batch heterogeneity. In consequence, the quality of these products, which is related to the reproducibility of their mass distribution pattern, should be addressed. Here, we propose an analytical approach to determine the peptide pattern as a quality attribute of Colagenart®, a product containing collagen hydrolysate. In addition, we evaluated the safety by measuring the viability of two cell lines exposed to the product. The consistency of peptide distribution was determined using Size Exclusion Chromatography (SEC), Mass Spectrometry coupled to a reversed pha

SUBMITTER: Lopez-Morales CA 

PROVIDER: S-EPMC6593667 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature