Kinetics of Mimivirus Infection Stages Quantified Using Image Flow Cytometry.

ABSTRACT: Due to the heterogeneity of viruses and their hosts, a comprehensive view of viral infection is best achieved by analyzing large populations of infected cells. However, information regarding variation in infected cell populations is lost in bulk measurements. Motivated by an interest in the temporal progression of events in virally infected cells, we used image flow cytometry (IFC) to monitor changes in Acanthamoeba polyphaga cells infected with Mimivirus. This first use of IFC to study viral infection required the development of methods to preserve morphological features of adherent amoeba cells prior to detachment and analysis in suspension. It also required the identification of IFC parameters that best report on key events in the Mimivirus infection cycle. The optimized IFC protocol en

SUBMITTER: Yaakov LB 

PROVIDER: S-EPMC6593739 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature