Resolvin D2 elevates cAMP to increase intracellular [Ca²⁺] and stimulate secretion from conjunctival goblet cells.

ABSTRACT: Under physiologic conditions, conjunctival goblet cells (CGCs) secrete mucins into the tear film to preserve ocular surface homeostasis. Specialized proresolving mediators (SPMs), like resolvins (Rvs), regulate secretion from CGCs and actively terminate inflammation. The purpose of this study was to determine if RvD2 stimulated mucin secretion and to investigate the cellular signaling components. Goblet cells were cultured from rat conjunctiva. Secretion was measured by an enzyme-linked lectin assay, change in intracellular [Ca²⁺] ([Ca²⁺]_i) using Fura-2, and cellular cAMP levels by ELISA. RvD2 (10^-11-10^-8 M) stimulated secretion, increased cellular cAMP levels and the [Ca²⁺]_i. RvD2-stimulated increase in [Ca²

SUBMITTER: Botten N 

PROVIDER: S-EPMC6593886 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature