Process monitoring of virus-like particle reassembly by diafiltration with UV/Vis spectroscopy and light scattering.

ABSTRACT: Virus-like particles (VLPs) have shown great potential as biopharmaceuticals in the market and in clinics. Nonenveloped, in vivo assembled VLPs are typically disassembled and reassembled in vitro to improve particle stability, homogeneity, and immunogenicity. At the industrial scale, cross-flow filtration (CFF) is the method of choice for performing reassembly by diafiltration. Here, we developed an experimental CFF setup with an on-line measurement loop for the implementation of process analytical technology (PAT). The measurement loop included an ultraviolet and visible (UV/Vis) spectrometer as well as a light scattering photometer. These sensors allowed for monitoring protein concentration, protein tertiary structure, and protein quaternary structure. The experimental setup was tested w

SUBMITTER: Rudt M 

PROVIDER: S-EPMC6593973 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature