Interaction between Brassica yellows virus silencing suppressor P0 and plant SKP1 facilitates stability of P0 in vivo against degradation by proteasome and autophagy pathways.

ABSTRACT: P0 protein of some polerovirus members can target ARGONAUTE1 (AGO1) to suppress RNA silencing. Although P0 harbors an F-box-like motif reported to be essential for interaction with S phase kinase-associated protein 1 (SKP1) and RNA silencing suppression, it is the autophagy pathway that was shown to contribute to AGO1 degradation. Therefore, the role of P0-SKP1 interaction in silencing suppression remains unclear. We conducted global mutagenesis and comparative functional analysis of P0 encoded by Brassica yellows virus (BrYV) (P0^Br ). We found that several residues within P0^Br are required for local and systemic silencing suppression activities. Remarkably, the F-box-like motif mutant of P0^Br , which failed to interact with SKP1, is destabilized in vivo. B

SUBMITTER: Li Y 

PROVIDER: S-EPMC6593998 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature