Bidirectional regulation between 1st trimester HTR8/SVneo trophoblast cells and in vitro differentiated Th17/Treg cells suggest a fetal-maternal regulatory loop in human pregnancy.

ABSTRACT:

Problem

During normal pregnancy, delicate crosstalk is established between fetus-derived trophoblasts and maternal immune cells to ensure maternal-fetal tolerance and successful placentation. Dysfunction in these interactions has been highly linked to certain pregnancy complications.

Method of study

Naïve CD4⁺ T cells were cultivated with or without 1st trimester derived trophoblast cell line HTR8/SVneo cells in the absence or presence of T helper 17 (Th17) or regulatory (Treg)cell-inducing differentiation conditions. After 5 days of co-culture, HTR8/SVneo cells and CD4⁺ T cells were harvested and analyzed using flow cytometry.

Results

CD4⁺ T cells exposed to HTR8/SVneo cells showed enhanced induction of CD4⁺ Foxp3⁺

SUBMITTER: Wang S 

PROVIDER: S-EPMC6594139 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature