Monitoring Neuronal Survival via Longitudinal Fluorescence Microscopy.

ABSTRACT: Standard cytotoxicity assays, which require the collection of lysates or fixed cells at multiple time points, have limited sensitivity and capacity to assess factors that influence neuronal fate. These assays require the observation of separate populations of cells at discrete time points. As a result, individual cells cannot be followed prospectively over time, severely limiting the ability to discriminate whether subcellular events, such as puncta formation or protein mislocalization, are pathogenic drivers of disease, homeostatic responses, or merely coincidental phenomena. Single-cell longitudinal microscopy overcomes these limitations, allowing the researcher to determine differences in survival between populations and draw causal relationships with enhanced sensitivity. This video gu

SUBMITTER: Weskamp K 

PROVIDER: S-EPMC6594158 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature